Sophisticated Analytical Instrument Facility

Sophisticated Analytical Instrument Facility

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Sophisticated Analytical Instrument Facility

The PacBio RS II is based on novel Single-Molecule, Real-Time (SMRT) technology, which enables the observation of DNA synthesis by a DNA polymerase in real time. Sequencing occurs on SMRT Cells, each containing thousands of Zero-Mode Waveguides (ZMWs) in which polymerases are immobilized. The ZMWs provide a window for watching the DNA polymerase as it performs sequencing by synthesis. The PacBio RS II sequencing provides average read lengths in excess of >10 kb with ultra-long reads > 40 kb. The long reads are characterized by high 99.999% consensus accuracy, and are ideal for assembly, targeted sequencing applications, scaffolding and spanning structural rearrangements. The PacBio Sequencing system includes an integrated software solution from beginning to end to support the key benefits of SMRT sequencing data: extraordinarily long reads, extremely high accuracy, exquisite sensitivity to variants, and unique epigenetic and methylation detection. The PacBio RS II sequencing system allows scientists to rapidly and cost effectively generate finished genome assemblies, Full-length sequencing of intact transcripts, reveal and understand epigenomes, and characterize genomic variation. It achieves the industry’s longest read lengths and highest consensus accuracy.

ICAR-NBFGR has 8-capillary ABI 3500 Genetic Analyzer (Applied Biosystems™). The DNA sequencer is used for sequencing PCR products upto 800 bases read length accurately. It employs BigDye™ Cycle Sequencing Kit, having Fluorescent labelled dideoxy-Nucleoside triphosphates (ddNTPs) for chain termination. For fragment analysis, 5 fluorescent dyes can detect simultaneously for multiplexing in high throughput fragment analysis applications.

The ROCHE MagNA Pure LC 2.0 Instrument is a tabletop instrument for rapid, cross-contamination-free preparation of nucleic acids and PCR setup. Equipped with a reliable robotic system, it can process up to 32 different samples in one batch from a wide variety of sources.. The magnetic-bead technology, combined with dedicated kits, enables fast isolation and easy setup and consistent isolation of high-quality DNA or RNA.

NRFC has 50 cell lines from fish species and these cell lines are available to researchers for their programmes. The NRFC also deposits the cell lines of fish origin developed by different researchers. The exchange of cell lines is implemented through material transfer agreement.

Fully automated system for identification of Gram positive and Gram Negative bacteria based on utilisation of 45 substrates. Facility to be operational by end of January 2018.

ASHAA, the AdvASHAA, the Advanced Supercomputing Hub for Aquatic Animals has been established at National Bureau of Fish Genetic Resources, Lucknow under NAIP funded subproject entitled 'Establishment of National Agricultural Bioinformatics Grid (NABG) in ICAR'. It is a hub for computational biology and bioinformatics to support frontier biotechnological research in fisheries domain. This includes integration of high performance system, attached network and parallel file system based storage. Further, this high performance computing system consists of hybrid hardware architecture of Linux and Windows based clusters i.e. Red hat Linux operating environment for HPC Systems and Window 7 for the workstations. Linkages with seven fisheries research tions under ICAR have been envisaged under this network which will help to bridge the gap of Omics information and knowledge within the fisheries domain. ASHAA will open up new vistas for molecular research in fisheries including genomics, transcriptomics, proteomics and metabolomics for enhancing the aquaculture production and development of improved varieties/strains. Hardware Configuration: 16 HPC Compute Nodes (for Linux based Cluster) Processor : 2x Intel Xeon Processor X5675, 3.07Ghz and minimum 6 Core/Processor
Memory: 96 GB DDR3 ECC 1333 MHz upgradable to 128 GB. (Minimum 8 GB module)
Hard Disk : Single 146GB 15K RPM SAS HDD. O.S: 64 bit Red Hat Enterprise Linux Operating System with unlimited user license.